Mastermind can be used to search for coding variants including: missense variants; insertion, deletion, and indel variants; nonsense variants; frameshift variants; and copy number variations (CNVs). Mastermind will also search for non-coding variants affecting 5’- and 3’-untranslated regions (UTRs), splice donor/acceptor sites, splice regions, introns, as well as intergenic variants up- and down-stream of neighboring genes.